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Fluorescence assay

" in MedChemExpress (MCE) Product Catalog:

By Targets:	Amyloid-β (1) Epigenetic Reader Domain (1) Fluorescent Dye (11) FXR (1) Histone Acetyltransferase (1) HSP (1) Isocitrate Dehydrogenase (IDH) (1) Liposome (2) MMP (2) Parasite (1) PARP (1) PD-1/PD-L1 (2) PGE synthase (1) Phosphodiesterase (PDE) (1) Ras (1)
By Research Areas:	Cancer (7) Inflammation/Immunology (6) Neurological Disease (1)
Click Chemistry:	Labeling and Fluorescence Imaging (1) Azide (1)

Inhibitors & Agonists

Fluorescent Dye

Biochemical Assay Reagents

Peptides

Click Chemistry

Targets Recommended: Biochemical Assay Reagents

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-130433

NBD Sphingosine	Fluorescent Dye	Others
NBD Sphingosine (NBD-Sph), a fluorochrome, is a fluorescence-labeled sphingosine. NBD Sphingosine can be uesd for fluorescence assay for sphingosine kinases .

HY-133705

Geldanamycin-FITC	HSP	Others
Geldanamycin-FITC, a Geldanamycin fluorescent probe, can be used in a fluorescence polarization assay for HSP90 inhibitors. Geldanamycin-FITC also can be used for detection of cell surface HSP90 .

HY-D1678

5(6)-Carboxynaphthofluorescein diacetate	Fluorescent Dye	Others
5(6)-Carboxynaphthofluorescein diacetate is a fluorescent substrate for esterase assays that can be cleaved by intracellular esterases, producing red fluorescence to measure enzyme activity (Ex = 590 nm; Em = 645 nm) .

HY-D1654

BDP 581/591 maleimide	Fluorescent Dye	Others
BDP 581/591 maleimide is a linker of the BDP 581/591 dye. It has a long fluorescence lifetime and can be used for fluorescence polarization assays. The maleimide group can react with thiol groups to form thioester bonds between pH 6.5 to 7.5, for the labeling of sulfhydryl groups of proteins and peptides.

HY-151775

BDP TR azide	Fluorescent Dye	Others
BDP TR azide is a click chemistry reagent containing an azide group that can react with alkynes, DBCO and BCN. BDP TR azide is also a fluorescent dye that can be used in fluorescence polarization assays and microscopy.

HY-12791

hPGDS-IN-1 1 Publications Verification	PGE synthase	Inflammation/Immunology
hPGDS-IN-1 is a hPGDS inhibitor ,with IC50 of 12 nM in the Fluorescence Polarization Assay or the EIA assay.

HY-18717

Mutant IDH1-IN-2 2 Publications Verification	Isocitrate Dehydrogenase (IDH)	Cancer
Mutant IDH1-IN-2 is a inhibitor of mutant Isocitrate dehydrogenase (IDH) proteins, with IC50 of in LS-MS biochemical assay, IC50 of 16.6 nM in Fluorescence biochemical assay.

HY-D0038

BTBCT	Fluorescent Dye	Inflammation/Immunology
BTBCT is mainly used as a label in time-resolved fluorescence immunoassays (TRFIA). The lower limit of detection for TSH TR-IFMA is 0.011 mIU/L in a 10 μl sample volume. The high fluorescence intensity and stability of BTBCT improves the sensitivity of the assay .

HY-131009

Fluorescein-NAD+	PARP	Others
Fluorescein-NAD+ is an alternative to radiolabeled NAD and a substrate for ADP-ribosylation. Fluorescein-NAD+ can be used in PARP assays by fluorescence microscopy. Extinction Coefficient: 262 nm.

HY-101027

GSK 4027 1 Publications Verification
GSK 4027 is a chemical probe for the PCAF/GCN5 bromodomain with an pIC₅₀ of 7.4±0.11 for PCAF in a time-resolved fluorescence resonance energy transfer (TR-FRET) assay.

HY-117468

Lissamine rhodamine B	Fluorescent Dye	Others
Lissamine rhodamine B is a red-fluorescent dye, it is a derivative of rhodamine. Lissamine rhodamine B can be used as a fluorescent probe to develop competitive aptamer fluorescence anisotropy/polarization (FA/FP) assays .

HY-134977

Atrovastatin-PEG3-FITC	Ras	Cancer
Atrovastatin-PEG3-FITC (compound S31) is a KRAS-PDEδ interaction inhibitor. Atrovastatin-PEG3-FITC acts as a ligand in fluorescence anisotropy assay .

HY-101027A

GSK4028 1 Publications Verification	Epigenetic Reader Domain Histone Acetyltransferase	Inflammation/Immunology Cancer
GSK4028 is the enantiomeric negative control of GSK4027, which is a PCAF/GCN5 bromodomain chemical probe, the pIC₅₀ of GSK4028 is 4.9 in a time-resolved fluorescence resonance energy transfer (TR-FRET) assay.

HY-157558

KDRLKZ-1 8SGE	Others	Others
KDRLKZ-1 is a KLHDC2 ligand (K_d = 0.36 μM), with IC₅₀s of 0.21 μM and 0.31 μM in alphaLISA and time-resolved fluorescence resonance energy transfer (TR-FRET) displacement assays .

HY-D1333

BDP 630/650 alkyne	Fluorescent Dye	Others
DP 630/650 alkyne is a red fluorescent dye with λ_excitation of 630 nM and λ_emission of 650 nM. The terminal alkyne group can be conjugated with various azides by copper catalyzed Click chemistry. BDP 630/650 alkyne can be used for fluorescence polarization assays .

HY-15941

5(6)-FITC 1 Publications Verification Fluorescein 5(6)-isothiocyanate; Fluorescein isothiocyanate 5- and 6- isomers	Fluorescent Dye	Others
5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA.

HY-D1657

BDP 581/591 azide	Fluorescent Dye	Others
BDP 581/591 azide is an azide derivative of BDP 581/591. BDP 581/591 is a universal, photostable fluorophore. BDP 581/591 azide can be used for the conjugation with both small molecules and biomolecules to construct tracers for fluorescence polarization assays and microscopy probes .

HY-19767A

GSK 3008348 hydrochloride 2 Publications Verification
GSK 3008348 hydrochloride is a small molecule integrin αvβ6 antagonist, extracted from patent WO/2014154725A1, example 1. The affinity (pIC50) for the human - a 6 protein in the Fluorescence Polarisation Assay for GSK 3008348 (hydrochloride) is 8.1, whereas its affinity in the cell Adhesion Assays was for: ανβ6 (pIC50 = 8.4); ανβ3 (pIC50 = 6); ανβ5 (pIC50 = 6.9); ανβ8 (pIC50 = 7.7).

HY-120635

BMS-1001 hydrochloride	PD-1/PD-L1	Inflammation/Immunology Cancer
BMS-1001 is an orally active human PD-L1/PD-1 immune checkpoint inhibitor. BMS-1001 exhibits low-toxicity in cells. The IC₅₀ value of BMS-1001 in a homogeneous time-resolved fluorescence (HTRF) binding assay is 2.25 nM .

HY-120647

BMS-1001	PD-1/PD-L1	Inflammation/Immunology Cancer
BMS-1001 is an orally active human PD-L1/PD-1 immune checkpoint inhibitor. BMS-1001 exhibits low-toxicity in cells. The IC₅₀ value of BMS-1001 in a homogeneous time-resolved fluorescence (HTRF) binding assay is 2.25 nM .

HY-139362

Enpp-1-IN-2	Phosphodiesterase (PDE)	Cancer
Enpp-1-IN-2 (Compound C) is a potent ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase 1) inhibitor with IC₅₀ values of 0.26, 0.48 and 2.0 μM evaluated by means of TG-mAMP, pNP-TMP, and ATP assays, respectively. TG (Tokyo Green)-mAMP: a newly synthesized sensitive ENPP1 fluorescence probe .

HY-153231

eGFP mRNA-LNP	Fluorescent Dye Liposome	Others
eGFP mRNA-LNP is a lipid nanoparticle (LNP) containing eGFP mRNA, suitable for assays of RNA delivery, translation efficiency, cell viability, etc. eGFP circRNA carries Enhanced Green Fluorescent Protein (Enhanced Green Fluorescent Protein) eGFP, which will express green fluorescent protein after entering the cell. eGFP is commonly used as a reporter gene detectable by fluorescence microscopy or flow cytometry .

HY-153232

eGFP circRNA-LNP	Fluorescent Dye Liposome	Others
eGFP circRNA-LNP is a lipid nanoparticle (LNP) containing eGFP circRNA, suitable for assays of RNA delivery, translation efficiency, cell viability, etc. eGFP circRNA carries Enhanced Green Fluorescent Protein (Enhanced Green Fluorescent Protein) eGFP, which will express green fluorescent protein after entering the cell. eGFP is commonly used as a reporter gene detectable by fluorescence microscopy or flow cytometry .

HY-117983

RU-505	Amyloid-β	Neurological Disease
RU-505 is an effective β-amyloid (Aβ)-fibrinogen interaction inhibitor with IC₅₀s of 5.00 and 2.72 μM in fluorescence polarization (FP) and AlphaLISA assays, respectively. RU-505 is highly permeable to the BBB. RU-505 reduces cerebral amyloid angiopathy (CAA). RU-505 can be used for the research of Alzheimer’s disease (AD) .

HY-153114

HEC96719	FXR	Inflammation/Immunology
HEC96719 is a selective and orally active tricyclic farnesoid X receptor (FXR) agonist with EC₅₀ values of 1.37 and 1.55 nM by time-resolved fluorescence energy transfer (TR-FRET) and luciferase reporter assays, respectively. HEC96719 significantly improves non-alcoholic steatohepatitis (NASH) and liver fibrosis with favorable tissue distribution in liver and intestine. HEC96719 can be used for the research of non-alcoholic steatohepatitis .

HY-163385

ALKBH1-IN-1	Others	Cancer
ALKBH1-IN-1 (Compound 13h) is a selective ALKBH1 inhibitor, with an IC₅₀ of 0.026 μM and 1.39 μM in the fluorescence polarization and enzyme activity assay, respectively. ALKBH1-IN-1 can modulate the level of DNA 6mA modifications. ALKBH1-IN-1 can be used to study the functions of ALKBH1 and DNA 6mA .

HY-P2185

NFF-3	MMP	Others
NFF-3, the peptide, is a selective MMP substrate. NFF-3 selectively binds to MMP-3 and MMP-10 to be hydrolyzed. NFF-3 is also cleaved by trypsin, hepatocyte growth factor activator, and factor Xa. Label NFF-3 with a CyDye pair, Cy3/Cy5Q, can produce fluorescence in cell assays to detect cell activity .

HY-P2185A

NFF-3 TFA	MMP	Others
NFF-3 TFA, the peptide, is a selective MMP substrate. NFF-3 TFA selectively binds to MMP-3 and MMP-10 to be hydrolyzed. NFF-3 TFA is also cleaved by trypsin, hepatocyte growth factor activator, and factor Xa. Label NFF-3 TFA with a CyDye pair, Cy3/Cy5Q, can produce fluorescence in cell assays to detect cell activity .

HY-D0952

Acridine Orange base	Parasite	Others
Acridine Orange base is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.) bright orange and, when used under appropriate conditions (pH=3.5, Ex=460 nm), distinguishes human cells in green for detection by fluorescence microscopy. Acridine Orange base fluoresces green when bound to dsDNA (Ex=488, Em=520-524) and red when bound to ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), also can be used in cell cycle assays .

Cat. No.	Product Name	Type

HY-130433

NBD Sphingosine

Dyes

NBD Sphingosine (NBD-Sph), a fluorochrome, is a fluorescence-labeled sphingosine. NBD Sphingosine can be uesd for fluorescence assay for sphingosine kinases .

NBD Sphingosine	Dyes
NBD Sphingosine (NBD-Sph), a fluorochrome, is a fluorescence-labeled sphingosine. NBD Sphingosine can be uesd for fluorescence assay for sphingosine kinases .

HY-133705

Geldanamycin-FITC	Fluorescent Dyes/Probes
Geldanamycin-FITC, a Geldanamycin fluorescent probe, can be used in a fluorescence polarization assay for HSP90 inhibitors. Geldanamycin-FITC also can be used for detection of cell surface HSP90 .

HY-D1678

5(6)-Carboxynaphthofluorescein diacetate	Fluorescent Dyes/Probes
5(6)-Carboxynaphthofluorescein diacetate is a fluorescent substrate for esterase assays that can be cleaved by intracellular esterases, producing red fluorescence to measure enzyme activity (Ex = 590 nm; Em = 645 nm) .

HY-D1654

BDP 581/591 maleimide	Fluorescent Dyes/Probes
BDP 581/591 maleimide is a linker of the BDP 581/591 dye. It has a long fluorescence lifetime and can be used for fluorescence polarization assays. The maleimide group can react with thiol groups to form thioester bonds between pH 6.5 to 7.5, for the labeling of sulfhydryl groups of proteins and peptides.

HY-151775

BDP TR azide	Fluorescent Dyes/Probes
BDP TR azide is a click chemistry reagent containing an azide group that can react with alkynes, DBCO and BCN. BDP TR azide is also a fluorescent dye that can be used in fluorescence polarization assays and microscopy.

HY-D0038

BTBCT	Dyes
BTBCT is mainly used as a label in time-resolved fluorescence immunoassays (TRFIA). The lower limit of detection for TSH TR-IFMA is 0.011 mIU/L in a 10 μl sample volume. The high fluorescence intensity and stability of BTBCT improves the sensitivity of the assay .

HY-15941

5(6)-FITC 1 Publications Verification Fluorescein 5(6)-isothiocyanate; Fluorescein isothiocyanate 5- and 6- isomers	Fluorescent Dyes/Probes
5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA.

HY-D1657

BDP 581/591 azide	Fluorescent Dyes/Probes
BDP 581/591 azide is an azide derivative of BDP 581/591. BDP 581/591 is a universal, photostable fluorophore. BDP 581/591 azide can be used for the conjugation with both small molecules and biomolecules to construct tracers for fluorescence polarization assays and microscopy probes .

HY-P2185

NFF-3	Dyes
NFF-3, the peptide, is a selective MMP substrate. NFF-3 selectively binds to MMP-3 and MMP-10 to be hydrolyzed. NFF-3 is also cleaved by trypsin, hepatocyte growth factor activator, and factor Xa. Label NFF-3 with a CyDye pair, Cy3/Cy5Q, can produce fluorescence in cell assays to detect cell activity .

HY-D0952

Acridine Orange base	Fluorescent Dyes/Probes
Acridine Orange base is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.) bright orange and, when used under appropriate conditions (pH=3.5, Ex=460 nm), distinguishes human cells in green for detection by fluorescence microscopy. Acridine Orange base fluoresces green when bound to dsDNA (Ex=488, Em=520-524) and red when bound to ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), also can be used in cell cycle assays .

Acridine Orange base

Fluorescent Dyes/Probes

Acridine Orange base is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.) bright orange and, when used under appropriate conditions (pH=3.5, Ex=460 nm), distinguishes human cells in green for detection by fluorescence microscopy. Acridine Orange base fluoresces green when bound to dsDNA (Ex=488, Em=520-524) and red when bound to ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), also can be used in cell cycle assays .

eGFP mRNA-LNP	Drug Delivery
eGFP mRNA-LNP is a lipid nanoparticle (LNP) containing eGFP mRNA, suitable for assays of RNA delivery, translation efficiency, cell viability, etc. eGFP circRNA carries Enhanced Green Fluorescent Protein (Enhanced Green Fluorescent Protein) eGFP, which will express green fluorescent protein after entering the cell. eGFP is commonly used as a reporter gene detectable by fluorescence microscopy or flow cytometry .

eGFP circRNA-LNP	Drug Delivery
eGFP circRNA-LNP is a lipid nanoparticle (LNP) containing eGFP circRNA, suitable for assays of RNA delivery, translation efficiency, cell viability, etc. eGFP circRNA carries Enhanced Green Fluorescent Protein (Enhanced Green Fluorescent Protein) eGFP, which will express green fluorescent protein after entering the cell. eGFP is commonly used as a reporter gene detectable by fluorescence microscopy or flow cytometry .

Cat. No.	Product Name	Target	Research Area

HY-P2185A

NFF-3 TFA	MMP	Others
NFF-3 TFA, the peptide, is a selective MMP substrate. NFF-3 TFA selectively binds to MMP-3 and MMP-10 to be hydrolyzed. NFF-3 TFA is also cleaved by trypsin, hepatocyte growth factor activator, and factor Xa. Label NFF-3 TFA with a CyDye pair, Cy3/Cy5Q, can produce fluorescence in cell assays to detect cell activity .

HY-P4725

Abz-Thr-Ile-Nle-p-nitro-Phe-Gln-Arg-NH2

Peptides Others

Abz-Thr-Ile-Nle-p-nitro-Phe-Gln-Arg-NH2 is a fluorogenic substrate, that can be used for the fluorescence screening assay .

Abz-Thr-Ile-Nle-p-nitro-Phe-Gln-Arg-NH2	Peptides	Others
Abz-Thr-Ile-Nle-p-nitro-Phe-Gln-Arg-NH2 is a fluorogenic substrate, that can be used for the fluorescence screening assay .

HY-P5908F

FAM-DEALA-Hyp-YIPD	Peptides	Others
FAM-DEALA-Hyp-YIPD is a fluorescent HIF-1α peptide, with the K_d of 180-560 nM. FAM-DEALA-Hyp-YIPD can be used to assess VHL binding in Fluorescence Polarization (FP) displacement assay, and evaluate the effect of VHL binding on degradation activity .

HY-P2185

NFF-3	MMP	Others
NFF-3, the peptide, is a selective MMP substrate. NFF-3 selectively binds to MMP-3 and MMP-10 to be hydrolyzed. NFF-3 is also cleaved by trypsin, hepatocyte growth factor activator, and factor Xa. Label NFF-3 with a CyDye pair, Cy3/Cy5Q, can produce fluorescence in cell assays to detect cell activity .

HY-P5415

DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS	Peptides	Others
DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS is a biological active peptide. (DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS is also called HIV protease substrate I in some literature. It is widely used for the continuous assay for HIV protease activity. The 11-kD protease (PR) encoded by the human immunodeficiency virus 1 (HIV-1) is essential for the correct processing of viral polyproteins and the maturation of infectious virus, and is therefore a target for the design of selective acquired immunodeficiency syndrome (AIDS) therapeutics. The FRET-based fluorogenic substrate is derived from a natural processing site for HIV-1 PR. Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that is linearly related to the extent of substrate hydrolysis. The fluorescence quantum yields of the HIV-1 PR substrate in the FRET assay increased by 40.0- and 34.4-fold, respectively, per mole of substrate cleaved. Because of its simplicity and precision in the determination of reaction rates required for kinetic analysis, this substrate offers many advantages over the commonly used HPLC or electrophoresis-based assays for peptide substrate hydrolysis by retroviral PRs. Abs/Em = 340nm/490nm.)

HY-P5510

Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-ψ-(COO)Ala-Ser-Lys(DABCYL)-NH2 HCV NS3 protease substrate	Peptides	Others
Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-ψ-(COO)Ala-Ser-Lys(DABCYL)-NH2 (HCV NS3 protease substrate) is a biological active peptide. (This peptide is a HCV protease substrate incorporating an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm.)

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